Typical serological response in acute Q fever
Infection:
- IgM and then IgG antibodies develop to phase II antigens in 10 to 14 days from symptom onset.
- Seroconversion or a fourfold rise in phase II IgG or CFT titre in convalescent serum is diagnostic of Q fever.
Abbreviations:
- CFT - complement fixation test
- EIA - enzyme immunoessay
- IFA - immunofluorescence assay
- PCR- polymerase chain reaction
- Ph - phase
*by IFA
Reproduced with permission of the copyright owners and authors Dr Jenny Robson, Sullivan Nicolaides Pathology and Dr Alex Chaudhuri, Director of Infectious Diseases, The Prince Charles Hospital, Chermside QLD 4032 and Senior Lecturer, University of Queensland School of Medicine.
Source: Medicine Today April 2013; 14(4): 54-59.
Serological patterns and PCR results for acute Q fever and chronic Q fever
Acute resolving Q fever
PCR
Serum |
positive |
Negative |
Negative |
Negative |
Negative |
---|
EIA
IgM - Phase 2 |
+- Positive |
Positive |
Equivocal* |
Negative |
Negative |
IgG - Phase 2 |
Negative |
Positive |
Positive |
Positive |
Positive** |
---|
CFT
CFT - Phase 2 |
<8 |
64 |
512 |
256 |
64 |
CFT - Phase 1 |
<8 |
<8 |
16 |
32 |
16 |
IFA
IgM - Phase 2 |
10-80 |
320 |
640 |
160 |
64 |
IgG - Phase 2 |
<10 |
320 |
640 |
1280 |
640 |
IgA - Phase 2** |
<10 |
<10 |
<10 |
40 |
20 |
IgM - Phase 1 |
<10 |
80 |
320 |
160 |
<10 |
IgG - Phase 1 |
<10 |
<10 |
320 |
160 |
160 |
IgA - Phase 1** |
<10 |
<10 |
<10 |
<10 |
<10 |
*May persist for months to years and not clinically significant
**May become undetectable over a long period of time
***Not always performed - clinical relevance uncertain
Reproduced with permission of the copyright owner and author Dr Jenny Robson, Sullivan Nicolaides Pathology.
Serology compatible with evolving Chronic Q fever
PCR
Serum/tissue |
Positive |
+-Positive* |
+-Positive* |
Nil. |
Nil. |
---|
CFT
CFT - Phase 2 |
<8 |
64 |
512 |
1028 |
512 |
CFT - Phase 1 |
<8 |
<8 |
512 |
1028 |
1028 |
IFA
IgM - Phase 2 |
10-80 |
320 |
640 |
160 |
<10 |
IgG - Phase 2** |
<10 |
320 |
1024 |
2560 |
10240 |
IgA - Phase 2 |
<10 |
<10 |
<10 |
40 |
20 |
IgM - Phase 1 |
<10 |
160 |
640 |
160 |
<10 |
IgG - Phase 1** |
<10 |
<10 |
1024 |
2560 |
20480 |
IgA - Phase 1*** |
<10 |
<10 |
<10 |
160 |
160 |
*Poor sensitivity in serum ~50%
**Persisting pahse 1 and to a lesser extent phase 2 IgG (CFT and IFA)
***Elevated phase 1 IgA > 160 may be present in chronic infection
Reproduced with permission of the copyright owner and author Dr Jenny Robson, Sullivan Nicolaides Pathology.